trap reaction solution Search Results


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Kementec Solutions Inc tmb x tra
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GE Healthcare pd spin trap g 25
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Thermo Fisher gene exp slc39a13 rn01485759 m1
Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, <t>Zip13,</t> Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
Gene Exp Slc39a13 Rn01485759 M1, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Varian Medical saturn 2000 ion trap mass spectrometer
Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, <t>Zip13,</t> Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
Saturn 2000 Ion Trap Mass Spectrometer, supplied by Varian Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bruker Corporation ion trap ms apparatus
Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, <t>Zip13,</t> Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
Ion Trap Ms Apparatus, supplied by Bruker Corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Carl Roth GmbH sodium acetate
Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, <t>Zip13,</t> Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
Sodium Acetate, supplied by Carl Roth GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Shanghai Guoda Pharmaceutical Co Ltd 125 w mercury lamp
Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, <t>Zip13,</t> Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
125 W Mercury Lamp, supplied by Shanghai Guoda Pharmaceutical Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher trap reaction solution
Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, <t>Zip13,</t> Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
Trap Reaction Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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GE Healthcare his trap hp
Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, <t>Zip13,</t> Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
His Trap Hp, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, Zip13, Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.

Journal: Journal of Clinical Biochemistry and Nutrition

Article Title: Dietary zinc supplementation increased TNFα and IL1β-induced RANKL expression, resulting in a decrease in bone mineral density in rats

doi: 10.3164/jcbn.15-71

Figure Lengend Snippet: Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, Zip13, Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.

Article Snippet: For real time PCR, the reaction mixture was prepared using the TaqMan Gene Expression Master Mix (Applied Biosystems, Foster, CA) with TaqMan gene expression assays (Applied Biosystems) for the following rat genes: SLC39A1 (Zip1) (Assay ID: Rn01458936_g1), SLC39A13 (Zip13) (Assay ID: Rn01485759_m1), SLC39A14 (Zip14) (Assay ID: Rn01468336_m1), SLC30A5 (ZnT5) (Assay ID: Rn01493867_m1), SLC30A7 (ZnT7) (Assay ID: Rn01518625_m1), metallothionein 1a (Mt1a) (Assay ID: Rn00821759_g1), metallothionein 2A (Mt2A) (Assay ID: Rn01536588_gH), SLC11A2 (divalent metal transporter 1 (DMT1)) (Assay ID: Rn01533109_m1), osteoprotegerin (OPG) (Assay ID: Rn00563499_m1), runt-related transcription factor 2 (Runx2) (Assay ID: Rn01512298_m1), Osterix (Assay ID: Rn02769744_s1), osteocalcin (Assay ID: Rn00566386_g1), alkaline phosphatase (ALP) (Assay ID: Rn01516028_m1), tumor necrosis factor α (TNFα) (Assay ID: Rn01525859_g1), interleukin 1 beta (IL1β) (Assay ID: Rn00580432_m1), receptor for activator of nuclear factor-κB (NF-κB) ligand (RANKL) (Assay ID: Rn00589289_m1), tumor necrosis factor receptor-associated factor 6 (TRAF6) (Assay ID: Rn01512911_m1), nuclear factor of activated T cells cytoplasmic 1 (NFATc1) (Assay ID: Rn04280453_m1), cathepsin K (CTSK) (Assay ID: Rn00580723_m1), tartrate-resistant acid phosphatase (TRAP) (Assay ID: Rn00569608_m1), and β actin (Assay ID: Rn00667869_m1).

Techniques: Expressing, Quantitative RT-PCR, Control, Gene Expression