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Image Search Results
Journal: Journal of Clinical Biochemistry and Nutrition
Article Title: Dietary zinc supplementation increased TNFα and IL1β-induced RANKL expression, resulting in a decrease in bone mineral density in rats
doi: 10.3164/jcbn.15-71
Figure Lengend Snippet: Effect of dietary zinc supplementation on the mRNA expression of zinc transporters, an iron transporter, and metallothioneins in rat femur. The mRNA expression of the zinc transporters, Zip1, Zip13, Zip14, ZnT5 and ZnT7, the iron transporter DMT1, and the metallothioneins Mt1a, and Mt2A were determined using qRT-PCR. The ordinate axis indicates the relative amount of mRNA compared with rats fed control diets. Gene expression levels were normalized with β-actin. Values are expressed as means ± SEM ( n = 6). Means with different letters differ significantly; p <0.05.
Article Snippet: For real time PCR, the reaction mixture was prepared using the TaqMan Gene Expression Master Mix (Applied Biosystems, Foster, CA) with TaqMan gene expression assays (
Techniques: Expressing, Quantitative RT-PCR, Control, Gene Expression